Mycobacterium tuberculosis Complex DNA from an Extinct Bison Dated 17,000 Years before the Present

摘要: In order to assess the presence of tuberculosis in Pleistocene bison and origin North America, 2 separate DNA extractions were performed by laboratories on samples from metacarpal an extinct long-horned that was radiocarbon dated at 17,870 ± 230 years before present had pathological changes suggestive tuberculosis. Polymerase chain reaction amplification isolated fragments DNA, which sequenced, spoligotyping also help determine its relationship various members Mycobacterium complex. Extensive precautions against contamination with modern M. complex employed, including analysis paleontologic specimens geographically laboratories.