A simple fluorimetric method for the estimation of free 11-hydroxycorticoids in human plasma.

作者: D. Mattingly

DOI: 10.1136/JCP.15.4.374

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摘要: A simple fluorimetric method is described for measuring free 11-hydroxycorticoids in human plasma. Only 2 ml. of plasma required each estimation and the fluorescence read a standard direct reading fluorimeter. Six estimations can be completed one half hours. It thus compares favourably with methods current use estimating urinary steroids, has added advantage not being dependent on accurate collection 24-hour urine samples.

参考文章(17)
Philip K. Bondy, Denis Abelson, James Scheuer, Theodore K.L. Tseu, Virginia Upton, Hilda Abelson, Audrey Clarke-Lewis, DETERMINATION OF CORTISOL IN HUMAN PLASMA BY QUANTITATIVE PAPER CHROMATOGRAPHY Journal of Biological Chemistry. ,vol. 224, pp. 47- 55 ,(1957) , 10.1016/S0021-9258(18)65009-4
Nicolas Zenker, Donald E. Bernstein, The estimation of small amounts of corticosterone in rat plasma. Journal of Biological Chemistry. ,vol. 231, pp. 695- 701 ,(1958) , 10.1016/S0021-9258(18)70434-1
Ian E. Bush, Avery A. Sandberg, Adrenocortical hormones in human plasma. Journal of Biological Chemistry. ,vol. 205, pp. 783- 793 ,(1953) , 10.1016/S0021-9258(18)49222-8
H. BRAUNSBERG, V. H. T. JAMES, THE DETERMINATION OF ADRENOCORTICAL STEROIDS IN BLOOD Journal of Endocrinology. ,vol. 21, pp. 327- 340 ,(1960) , 10.1677/JOE.0.0210327
R. E. Peterson, Aurora. Karrer, S. L. Guerra, Evaluation of Silber-Porter Procedure for Determination of Plasma Hydrocortisone Analytical Chemistry. ,vol. 29, pp. 144- 149 ,(1957) , 10.1021/AC60121A042
HENRY B. HALE, CLYDE H. KRATOCHVIL, JAMES P. ELLIS, EDGAR W. WILLIAMS, PLASMA CORTICOSTEROID LEVELS IN AIRCREWMEN AFTER LONG FLIGHTS The Journal of Clinical Endocrinology and Metabolism. ,vol. 18, pp. 1441- 1443 ,(1958) , 10.1210/JCEM-18-12-1440
C.P. Stewart, F. Albert-Recht, Laila M. Osman, The simultaneous fluorimetric microdetermination of cortisol and corticosterone in plasma Clinica Chimica Acta. ,vol. 6, pp. 696- 707 ,(1961) , 10.1016/0009-8981(61)90116-4
DON H. NELSON, LEO T. SAMUELS, A method for the determination of 17-hydroxycorticosteroids in blood; 17-hydroxycorticosterone in the peripheral circulation. The Journal of Clinical Endocrinology and Metabolism. ,vol. 12, pp. 519- 526 ,(1952) , 10.1210/JCEM-12-5-519