Hydrolysis of a mixture of saccharides by cellulase from Aspergillus niger and its application for visible-light-induced hydrogen gas production system using Mg chlorophyll-a and platinum nanoparticles
作者： Yutaka Amao , Takamasa Hirakawa
DOI: 10.1016/J.IJHYDENE.2010.04.030
关键词:
摘要: Abstract Cellulase obtained from Aspergillus niger was used to hydrolyze a mixture of saccharides containing sucrose, maltose, and cellobiose; the reduced form nicotinamide-adenine dinucleotide (NAD+), which is NADH, produced during hydrolysis in presence NAD+ glucose dehydrogenase (GDH). We have developed visible-light-induced enzymatic biohydrogen production system involving combination cellulase-mediated hydrogen by platinum nanoparticles using photosensitization Mg chlorophyll-a (Mg Chl-a). Continuous gas observed when reaction saccharides, cellulase, GDH, NAD+, Chl-a, methylviologen (MV2+, an electron donor), irradiated visible light. After 120 min irradiation, amount approximately 2.8 μmol.
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