Structural rearrangements of the ribosome at the tRNA proofreading step
作者： Lasse Jenner , Natalia Demeshkina , Gulnara Yusupova , Marat Yusupov , None
DOI: 10.1038/NSMB.1880
关键词:
摘要: Discrimination of tRNA on the ribosome occurs in two consecutive steps: initial selection and proofreading. Here we propose a proofreading mechanism based comparison crystal structures 70S with an empty A site or occupied by uncharged cognate near-cognate tRNA. We observe that ribosomal proteins S13, S19, L16, L25, L27 L31 are actively involved suggest begins monitoring entire anticodon loop nucleotides from 16S rRNA (helices 18 44) small subunit 23S (helix 69) large involvement magnesium ions. Subsequently, elbow region is scanned 38 89) determining whether to accommodate acceptor end peptidyl transferase center not.
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