Cloning, expression and characterization of a novel (2R,3R) -2,3-butanediol dehydrogenase from Bacillus thuringiensis
作者： Feng-Qin Gong , Qi-Shun Liu , Hai-Dong Tan , Tang Li , Cheng-Yu Tan
DOI: 10.1016/J.BCAB.2019.101372
关键词:
摘要: Abstract Acetoin (3-hydroxy-2-butanone) is an important four-carbon compound widely used in the food industry and other industrial applications. This study aimed to identify a novel butanediol dehydrogenase that can efficiently catalyze formation of acetoin. A dehydrogenase, BtBDH, was identified from Bacillus thuringiensis subsp. Kurstaki ACCC 10066, its enzymatic properties were characterized. The optimum pH temperature for oxidation activity BtBDH 10.0 50 °C, respectively, those reduction activities 7.5 35 °C, respectively. In addition, it exhibits stability over wide range (6–10) temperatures up 70 °C. showed good after storage 3 months at 4 °C. Moreover, ethylenediaminetetracetic acid (EDTA) inhibits indicating enzyme metal-dependent. characterized (2R,3R) −2,3-butanediol dehydrogenase. Its excellent ensure great application potential production
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