Sildenafil promotes adipogenesis through a PKG pathway
作者： Xiaodong Zhang , Jun Ji , Guirui Yan , Jingwei Wu , Xiaoyun Sun
DOI: 10.1016/J.BBRC.2010.05.064
关键词:
摘要: Sildenafil is the first oral PDE5 inhibitor for treatment of erectile dysfunction and pulmonary arterial hypertension. In present study, we investigated effect sildenafil on adipogenesis in 3T3L1 preadipocytes. Treatment with 8 days significantly promoted characterized by increased lipid droplet triglyceride content cells. Meanwhile, induced a pronounced up-regulation expression adipocyte-specific genes, such as aP2 GLUT4. The results RT-PCR Western blotting further showed that sequential C/EBP beta, PPAR gamma alpha. Additionally, found other two inhibitors (vardenafil tadalafil) cGMP analog 8-pCPT-cGMP also adipogenesis. Likewise, could up-regulate adipogenic genes. Importantly, PKG Rp-8-pCPT-cGMP was able to inhibit both 8-pCPT-cGMP-induced Furthermore, basal insulin-mediated glucose uptake cells, which counteracted Rp-8-pCPT-cGMP. These indicate promote accompanied through pathway at least partly.
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