Enzymic creatinine assay: a new colorimetric method based on hydrogen peroxide measurement.

摘要: We describe a new colorimetric method for measuring creatinine in serum and urine. Creatinine hydrolysis is catalyzed by amidohydrolase, the creatine so produced assayed reactions sequentially amidinohydrolase sarcosine oxidase system that generates hydrogen peroxide. The peroxide measured at 510 nm reaction horseradish peroxidase, with 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone as chromogen. This series of complete 30 min room temperature. A blank sample measurement corrects endogenous creatine. standard curve linear concentrations great 2.21 mmol/L. Analytical recovery human sera urine averaged 99.8%. Within-run between-run precision studies gave CVs less than or equal to 3.3 4.3% concentration 69 mumol/L. Results this agree well (r greater 0.99) those both enzymic ultraviolet Wahlefeld fuller's earth/Jaffe method.