Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro

摘要: Mycoplasma hyopneumoniae, the agent of porcine enzootic pneumonia (EP), is able to persist in lung tissue and evade destruction by host for several weeks. To understand mechanism pathogen survival, phagocytic uptake M. hyopneumoniae primary alveolar macrophages was investigated. Intracellular location survival were explored using gentamicin assays, flow cytometry confocal microscopy 232 labelled with green fluorescent protein (GFP). Following 1 h 16 h co-incubation, few viable recovered from inside macrophages. Flow cytometric analysis incubated expressing GFP indicated that mycoplasmas became associated macrophages, but shown be extracellular when actin-dependent phagocytosis blocked cytochalasin D. Confocal detected GFP-labelled numbers increased modestly time incubation. Neither addition serum complement or convalescent EP-recovered pigs enhance engulfment hyopneumoniae. This investigation suggests evades significant this may a immune escape respiratory tract.