A specific and sensitive assay for disulfides.
作者： W L Zahler , W W Cleland
DOI: 10.1016/S0021-9258(19)81724-6
关键词:
摘要: A specific and sensitive assay for disulfide groups has been developed, based on reduction with dithioerythritol or dithiothreitol determination of the resulting monothiols 5,5′-dithiobis(2-nitrobenzoic acid) in presence arsenite. The dissociation constants complexes formed between arsenite dithioerythritol, dithiothreitol, 1,3-dithioglycerol, 1,2-dithioglycerol have measured, as well rate formation breakdown bimolecular reaction acid). pK values are 8.3 9.5, those 9.0 9.9.
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