High phosphate feeding promotes mineral and bone abnormalities in mice with chronic kidney disease

摘要: Background Chronic kidney disease-mineral bone disorder (CKD-MBD) is a systemic syndrome characterized by imbalances in mineral homeostasis, renal osteodystrophy (ROD) and ectopic calcification. The mechanisms underlying this individuals with chronic disease (CKD) are not yet clear. Methods We examined the effect of normal phosphate (NP) or high (HP) feeding setting CKD on pathology, serum biochemistry vascular calcification calcification-prone dilute brown non-agouti (DBA/2) mice. Results In both NP HP-fed mice, elevated parathyroid hormone alkaline phosphatase (ALP) levels were observed, but phosphorus equivalent compared sham controls. mice diet showed trabecular alterations long consistent high-turnover ROD, including increased number abundant osteoblasts osteoclasts. Despite biochemical changes, CKD/NP did develop contrast, CKD/HP developed arterial medial (AMC), more severe cortical abnormalities that included decreased thickness density, porosity. Cortical porosity strongly correlated degree aortic Conclusions HP was required to induce full spectrum CKD-MBD symptoms