Conjunctival proinflammatory and proapoptotic effects of latanoprost and preserved and unpreserved timolol: an ex vivo and in vitro study.

摘要: PURPOSE. To compare the toxicity of latanoprost and preserved unpreserved timolol on conjunctival cells. Expression inflammatory markers MUC5AC-related mucin production were evaluated by impression cytology in a case-control ex vivo study. The proapoptotic effect same drugs was also vitro cell line compared with that benzalkonium chloride (BAC). METHODS. Impression (IC) specimens obtained from series normal subjects patients glaucoma treated for at least 1 year eye drops or timolol. All groups comparable age duration treatment. HLA-DR, intercellular adhesion molecule (ICAM)-1, masked manner flow cytometry. For study, human conjunctiva-derived 0.02% BAC-containing timolol, BAC alone 15 minutes, followed not 4 24 hours recovery medium. Cell viability chromatin condensation using microplate cold light cytofluorometry neutral red Hoechst 33342 tests, respectively. Hoechst-neutral ratio defined apoptosis assay, cytoskeleton changes assessed confocal microscopy. RESULTS. No difference found between eyes those receiving Preserved significantly increased marker expression decreased MUC5AC expression, but to higher extent group latanoprost. In vitro, triggered apoptosis- however, lesser than did alone. Unpreserved cause any toxicity. CONCLUSIONS. These studies demonstrate exhibit proinflammatory effects cells does Latanoprost caused less toxicity, both toxic results suggest potential protective prostaglandin analogue against