Pulsed electromagnetic field stimulation of MG63 osteoblast-like cells affects differentiation and local factor production.

摘要: Pulsed electromagnetic field stimulation has been used to promote the healing of chronic nonunions and fractures with delayed healing, but relatively little is known about its effects on osteogenic cells or mechanisms involved. The purpose this study was examine response osteoblast-like a pulsed signal clinically determine if modulates production autocrine factors associated differentiation. Confluent cultures MG63 human were placed between Helmholtz coils exposed consisting burst 20 pulses repeating at 15 Hz for 8 hours per day 1, 2, 4 days. Controls cultured under identical conditions, no applied. Treated control alternated two comparable incubators and, therefore, active coils; measurement temperature culture medium indicated that application did not generate heat above level found in incubator medium. caused reduction cell proliferation basis number [3H]thymidine incorporation. Cellular alkaline phosphatase-specific activity increased signal, maximum 1. In contrast, enzyme cell-layer lysates, which included phosphatase-enriched extracellular matrix vesicles, continued increase time exposure signal. After 1 2 days exposure, collagen synthesis osteocalcin greater than cultures. Prostaglandin E2 treated significantly reduced days, whereas transforming growth factor-β1 increased; treatment, however, levels both local similar those controls. results indicate enhanced differentiation as net effect fields osteoblasts, evidenced by decreased activity, synthesis, production. appears vesicles higher phosphatase layers isolated cells, commensurate factor-β1. osteoblasts are sensitive stimulation, alters through changes factor