Altered morphology and composition of Zymogen Granules in acute pancreatitis

摘要: ALTERED MORPHOLOGY AND COMPOSITION OF ZYMOGEN GRANULES IN ACUTE PANCREATITIS by AMANDA FLACK August 2014 Advisor: Dr. Bhanu Jena Major: Physiology Degree: Doctor of Philosophy In healthy physiology, pancreatic digestive enzymes secreted following a meal are stored as inactive zymogens within membrane-bound secretory vesicles called Zymogen Granules (ZG), and activated extracellularly. acute pancreatitis however, the prematurely cell, resulting in autodigestion tissue. Pancreatitis is gastrointestinal disorder which there over 200,000 hospitalizations per year with 5% mortality rate. It has been demonstrated that blocked from being cell leading to acinar surrounding tissue death. Little known about specific mechanism proteins lipids might participate this process. Here it reported pancreatitis, changes both proteome lipidome ZG, contributing altered ZG morphology function. Using EM AFM an increase size early 2h induction pancreatitis. LC-MS-based lipid protein profiling immunochemistry, collectively demonstrate volume activity regulating lipids,