Increased protein kinase C activity in low density eosinophils.

摘要: Eosinophil heterogeneity is expressed in cell density, membrane receptors and function. It has been observed that increases some functional activities correlate with decreased sedimentation density human eosinophils. However, the cellular mechanisms to explain up-regulation of eosinophil function have not fully explored. Protein kinase C (PKC) an important family enzymes mediating signal transduction for a wide variety functions many different types. Changes activity PKC could differences In these experiments, granulocyte lysate supernatants was measured as phosphatidyl serine-dependent transfer 32P from [gamma-32P]ATP protein substrate under conditions maximal stimulation; measure activatable concentration. We present eosinophils (87.2 +/- 8.4 pmol PO4 incorporated into histone per minute 10(6) cells, n = 30) significantly neutrophils assayed same (91.5 5.6 U, 31) but percent total serine dependent greater (97 1% vs 81 neutrophils, p 0.001). Blood isolated low Percoll fractions had higher (120 16 U) than found cells subjects (81 19 9, 0.011). When recovered bronchoalveolar lavage (BAL) fluid after segmental Ag challenges were assayed, BAL similar blood-derived equal tended be or cells. The beta isozyme alpha gamma detected by Western blotting isozyme-specific mAb. These data indicate primarily caused beta-isozyme, related may relationship