Cyclic AMP Receptor Protein Regulates cspE, an Early Cold-Inducible Gene, in Escherichia coli

摘要: ABSTRACT cspE , a member of the cspA family cold shock proteins in Escherichia coli, is an early cold-inducible protein. The nucleic acid melting ability and transcription antiterminator activity CspE have been reported to be critical for growth at low temperature. Here, we show that cyclic AMP receptor protein (CRP), global regulator involved sugar metabolism, upregulates E. coli. Sequence analysis upstream region revealed putative CRP target site centered −61.5 relative start. binding this was demonstrated using electrophoretic mobility shift assays. presence shown essential P activation by CRP. Mutational indicated intact second core motif more important than first CRP-P interaction. Based on promoter architecture, classified as class I CRP-dependent promoter. This further substantiated our data demonstrating involvement AR1 domain transcription. Furthermore, substitutions key residues RNA polymerase α-subunit C-terminal (α-CTD), which are transcription, showed 265 287 determinants In addition, deletion crp led defect temperature, suggesting plays role adaptation.