Lysophosphatidylcholine enhances superoxide anions production via endothelial NADH/NADPH oxidase.
作者： Saori Takeshita , Nobutaka Inoue , Dayaun Gao , Yoshiyuki Rikitake , Seinosuke Kawashima
关键词:
摘要: Reactive oxygen species (ROS) including superoxide anions (O2(-)) play a key role in atherogenesis, and endothelial cells have the ability to generate ROS. To investigate enzymatic sources of ROS effects lysophosphatidylcholine (LPC), an atherogenic lipid, we measured production cultured bovine aortic (BAECs) by lucigenin-enhanced chemiluminescence (CL) method electron spin resonance (ESR). BAEC homogenates had activity NADH/NADPH oxidase. BAECs on microcarrier beads generated O2(-) under basal conditions. The inhibition NADH/ NADPH oxidase diphenylene iodonium (DPI) significantly attenuated production, whereas no inhibitors other oxidases suppressed it. Although LPC enhanced approximately 3.1-fold, its action was DPI. Tyrosine kinase LPC-induced production. ESR with DMPO demonstrated that increased formation DMPO-hydroxyl adduct dose- time-dependent manners. These data suggest is mainly mediated system activates this enhance through tyrosine kinase-dependent pathway. enhancement probably involved property.
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