Cell biology of microbes and pharmacology of antimicrobial drugs explored by Atomic Force Microscopy

摘要: Antimicrobial molecules have been used for more than 50 years now and are the basis of modern medicine. No surgery can nowdays be imagined to performed without antibiotics; dreadful diseases like tuberculosis, leprosis, siphilys, broadly all microbial induced diseases, cured only through use antimicrobial treatments. However, situation is becoming complex because ability microbes adapt, develop, acquire, share mechanisms resistance agents. We choose introduce this review by briefly drawing panorama discovery development, but also emergence resistance. Then we describe how Atomic Force Microscopy (AFM) provide a better understanding action these drugs at nanoscale level on interfaces. In section, will address questions: (1) does drug treatment affect morphology single microbes?; (2) do modify nanomechanical properties microbes, or play role in activity efficiency?; (3) adhesive abilitites affected treatment? Finally, second part focus recent studies aimed changing paradigm molecule/cell technology that AFM typically represents. Recent work dealing with creation microbe array which explored presented, as developments constitute first steps toward transforming into higher throughput technology. discuss papers using NanoMechnanicalSensors (NEMS), demonstrate interest such approaches clinical microbiology detect quickly high accuracy