Tuning Cerium(IV)-Assisted Hydrolysis of Phosphatidylcholine Liposomes under Mildly Acidic and Neutral Conditions.

摘要: With the goal of designing a lysosomal phospholipase mimic, we optimized experimental variables to enhance Ce(IV) -assisted hydrolysis phosphatidylcholine (PC) liposomes. Our best result was obtained with chelating agent bis-tris propane (BTP). Similar hydrolytic enzyme, PC phosphate ester bonds higher at pH (∼4.8) compared 7.2. In presence BTP, average cleavage yield ∼pH 4.8 and 37 °C was: 67±1 %, 5.7-fold than 7.2 roughly equivalent percent phospholipid found on metal-accessible exo leaflet small No precipitation observed. When BTP absent, there significant turbidity, amount (69±1 %) 2.1-fold results show that generates homogenous solutions hydrolyze enhanced selectivity for pH.