High prevalence of RET rearrangement in thyroid tumors of children from Belarus after the Chernobyl reactor accident

摘要: RET rearrangement was studied in papillary thyroid carcinomas (PTC) of children exposed to radioactive fallout Belarus after the Chernobyl accident. To detect small tissue samples from thyroidectomy specimen (12 PTC children; 2 and 1 follicular carcinoma adults; non-tumorous 4 adults as controls), a RT-multiplex PCR developed using primers suited amplify fragments different quantities depending on presence or absence rearrangements tissues. The type determined by RT-PCR direct sequencing for ret/PTC1, 3. Two-thirds revealed rearrangement, with ret/PTC3 being more frequent factor 3 than ret/PTC1. ret/PTC2 not detected. All rearrangement-positive tumors had lymph node metastasis while half wild-type cRET not. More cases expressed only ELE/RET transcript expected, but also RET/ELE transcript. Intrachromosomal involving adjacent H4 ELE gene chromosome no. 10 is very event cancer Chernobyl-contaminated zone Belarus.