Inflammatory cytokine gene polymorphism profiles in Turkish patients with ulcerative colitis.

摘要: Aim To investigate IL-1α, IL-1β, IL-1R , IL-4RA, TGF-β, TNF-α and IFN-γ, genes polymorphism in Turkish patients with ucerative colitis (UC). Methods An analysis was carried out at Trabzon Karadeniz Technical University Medicine Faculty Gastroenterology polyclinics between March 2005 May 2011 on 51 patient UC (cases) 100 healthy individuals (controls). PCR-SSP cytokine gene panel (Helderberg kits) based techniques for of polymorphisms were used. Results Changes allelic frequencies each the investigated eight ulcerative found. Among analyzed here, highest statistically significant change observed position -308 G/A (339.7%). The following increases IL-IR mspa T/C variation (179.4%), IFN-γ 5644A/T (77.4%), IL-1β -511T/C SNPs (35.9% ). In other genes, changes found to be decreasing TGF- β codon10C / T (-71.9%), IL4RA + 1902G A (-47.3 %), IL- 1α -889T C (-37.7%). lowest negative (-25.9%) allele frequency 1β 3962T (p les than 0.000). addition, there genotypic seven polymophic site only one 3962TT/TC/CC not changed. Conclusion Genes is itself determining factor clinical diagnoses. However, it can used diagnosis order determine low level or high variations polymorphisms.