[3H](+)MK801 Radioligand Binding Assay at the N‐Methyl‐D‐Aspartate Receptor

摘要: The N-methyl-D-aspartate subtype of glutamate receptor is unusual in that it requires two endogenous agonists for activation. Thus, addition to glutamate, the amino acid glycine (or possibly D-serine) an essential co-agonist. This unit presents a radioligand binding protocol detects ligand activity at NMDA receptor-associated site. convenient approach exploits ability modulators alter kinetics ligands bind channel-blocking site receptor. takes advantage one most potent and specific this receptor, case [(3)H](+)MK801. Importantly, assay can detect differentiate antagonists A measurement with [(3)H](+)MK801 provided, along support protocols provide information aid design assays site, data analysis.