Nanovaccines for malaria using Plasmodium falciparum antigen Pfs25 attached gold nanoparticles

摘要: Malaria transmission-blocking vaccines (TBV) targeting sexual stages of the parasite represent an ideal intervention to reduce burden disease and eventual elimination at population level in endemic regions. Immune responses against stage antigens impair development inside mosquitoes. Target identified Plasmodium falciparum include surface proteins Pfs230 Pfs48/45 male female gametocytes Pfs25 expressed zygotes ookinetes. The latter has undergone extensive evaluation pre-clinical phase I clinical trials remains one leading target for TBV. a complex tertiary structure characterized by four EGF-like repeat motifs formed 11 disulfide bonds, it been rather difficult obtain as homogenous product native conformation any heterologous expression system. Recently, we have reported codon-harmonized recombinant Escherichia coli (CHrPfs25) which elicited highly potent malaria antibodies mice. In current study, investigated CHrPfs25 along with gold nanoparticles different shapes, size physicochemical properties adjuvants induction transmission blocking immunity. results revealed that delivered various strong suggested based formulations can be developed nanovaccines enhance immunogenicity vaccine antigens.