The apical complex protein Pals1 is required to maintain cerebellar progenitor cells in a proliferative state

摘要: Through their biased localization and function within the cell, polarity complex proteins are necessary to establish cellular asymmetry required for tissue organization. Well-characterized germinal zones, mitogenic signals cell types make cerebellum an excellent model addressing crucial of in generation organization neural tissues. Deletion apical protein Pals1 developing results a remarkably undersized with disrupted layers poorly formed folia strikingly reduced granule production. We demonstrate that is not only essential organogenesis, but also preventing premature differentiation thus maintaining progenitor pools cerebellar including neuron precursors external layer. In mouse mutants, expression genes regulate cycle was diminished, correlating loss proliferating population zones. Furthermore, enhanced Shh signaling through activated Smo cannot overcome impaired generation, arguing epistatic role proliferation capacity. Our study identifies as novel intrinsic factor regulates cells deficiency potential inhibitor overactive signaling.