Oxidative Stress Induced Age Dependent Meibomian Gland Dysfunction in Cu, Zn-Superoxide Dismutase-1 (Sod1) Knockout Mice

摘要: Purpose The purpose of our study was to investigate alterations in the meibomian gland (MG) Cu, Zn-Superoxide Dismutase-1 knockout (Sod1−/−) mouse. Methods Tear function tests [Break up time (BUT) and cotton thread] ocular vital staining test were performed on Sod1−/− male mice (n = 24) aged 10 50 weeks, age sex matched wild–type (+/+) 25). Tear serum samples collected at sacrifice for inflammatory cytokine assays. MG specimens underwent Hematoxylin Eosin staining, Mallory fibrosis, Oil Red O lipid TUNEL immunohistochemistry stainings 4HNE, 8-OHdG CD45. Transmission electron microscopic examination (TEM) also performed. Results Corneal scores significantly higher compared with wild type throughout follow-up. IL-6 TNF-α levels showed significant elevations week mice. an accumulation large droplets weeks. Immunohistochemistry revealed both increased oxidative stress marker acinar epithelium CD45 increasing cell infiltrates from weeks TEM prominent mitochondrial changes mice. Conclusions Our results suggest that reactive oxygen species might play a role pathogensis dysfunction. The mouse appears be promising model associated alterations.