The mechanism of oleanolic acid monoglycosides transport into vacuoles isolated from Calendula officinalis leaf protoplasts

摘要: Abstract The uptake of [3- 3 H]-oleanolic acid 3- O -monoglucoside and -monoglucuronide into vacuoles isolated from Calendula officinalis leaf protoplasts was investigated under various conditions. transport monoglucoside stimulated by ATP pyrophosphate, sensitive to protein-modifying agent as well dissipaters the membrane potential. On contrary, monoglucuronide not dependent on tonoplast energization affected only a factor. Moreover, kinetics both monoglycosides characterized concentration-dependent with saturation phase. obtained results indicate that oleanolic is transported an active, carrier-mediated energy-dependent mechanism, whereas passive, process.