Omentin-1, a novel adipokine, is decreased in overweight insulin-resistant women with polycystic ovary syndrome: ex vivo and in vivo regulation of omentin-1 by insulin and glucose.

摘要: OBJECTIVE— Polycystic ovary syndrome (PCOS) is associated with insulin resistance and obesity. Recent studies have shown that plasma omentin-1 levels decrease Currently, no data exist on the relative expression regulation of in adipose tissue women PCOS. The objective this study was to assess mRNA protein omentin-1, including circulating omental PCOS matched control subjects. Ex vivo also studied. RESEARCH DESIGN AND METHODS— Real-time RT-PCR Western blotting were used omentin-1. Plasma measured by enzyme-linked immunosorbent assay. effects d-glucose, insulin, gonadal adrenal steroids analyzed ex vivo. (hyperinsulinemia) assessed a prolonged insulin-glucose infusion. RESULTS— In addition decreased ( P < 0.05), compared subjects, there significantly lower 0.01) 0.05) 0.01). Furthermore, explants, glucose dose-dependently expression, levels, secretion into conditioned media 0.05, Also, hyperinsulinemic induction healthy subjects reduced 0.01). CONCLUSIONS— Our novel findings reveal downregulated glucose. These may, part, explain observed our overweight