Studies on the methanolysis of small amounts of purified phospholipids for gas chromatographic analysis of fatty acid methyl esters
作者： K. Eder , A.M. Reichlmayr-Lais , M. Kirchgessner
DOI: 10.1016/0021-9673(92)87054-C
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摘要: Abstract The methanolysis of small amounts purified phosphoglycerides and sphingomyelin was studied a quantitative comparison five methods for the standard made. These were based on with boron trifluoride-methanol, methanolic sodium methoxide (at ambient temperature heating) sulphuric acid. A further method saponification hydroxide subsequent esterification trifluoride- methanol. Under experimental conditions, only methoxide-catalysed at gave complete phosphoglycerides. For sphingomyelin, trifiuoride-methanol, acid hydrochloric used. It found that takes 15 h 90°C. Based these results, procedures separated by high-performance liquid chromatography are presented.
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