Methods and compositions for multiplex amplification of nucleic acids
作者： Hajime Matsuzaki , Eric A. Murphy
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摘要: A method is described for predetermining ratios of primer pairs present in a single reaction vessel so as to achieve approximately equimolar yield products. The are determined function the length amplicon and other amplicons being simultaneously tested. primers may desirably be p53 gene sequences.
google.nl 参考文章(10)
Russell G. Higuchi, Henry A. Erlich, Methods for nucleic acid amplification ,(1992)
John K. Stevens, Eleftherios Diamandis, James M. Dunn, Method, reagents and kit for diagnosis and targeted screening for p53 mutations ,(1995)
John Bruce C, William Harold C, John W. C, Marlene M. C, John W. H. C, Method for coamplification of two different nucleic acid sequences using polymerase chain reaction. ,(1994)
Anthony P. Shuber, Universal primer sequence for multiplex DNA amplification ,(1996)
Jan Vijg, Daizong Li, Method of and apparatus for diagnostic DNA testing Official Gazette of the United States Patent and Trademark Office Patents. ,vol. 1214, pp. 5297- 5298 ,(1996)
L A Haff, Improved quantitative PCR using nested primers. Genome Research. ,vol. 3, pp. 332- 337 ,(1994) , 10.1101/GR.3.6.332
Edwin Southern, Analysing polynucleotide sequences ,(2002)
Elizabeth R. Billyard, Daniel Louis Kacian, Nancy Lau Liu Shen, Sherrol H. McDonough, Frank R. Gonzales, Karen W. Shannon, Thomas Brendan Ryder, Maria R. Castillo, Richard C. Harvey, Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product ,(1995)
Sprecher Cynthia J, Lins Ann M, Schumm James W, MULTIPLEX AMPLIFICATION OF SHORT TANDEM REPEAT LOCMULTIPLEX AMPLIFICATION OF SHORT TANDEM REPEAT LOCI I ,(1997)