Generation of natural pH gradients in microfluidic channels for use in isoelectric focusing

摘要: As a part of an ongoing investigation the use isoelectric focusing (IEF) in microfluidic devices, pH gradients were electrochemically formed and optically quantified channels using acid−base indicators. The microchannels consisted two parallel 40-mm-long electrodes with interelectrode gap 2.54 mm; top bottom transparent windows separated by 0.2 mm. Gradients as result electrochemical decomposition water at applied potential not higher than 2.5 V to avoid generation gas bubbles. Solutions contained low concentrations single buffer. stability their sensitivity changes initial conditions investigated under static (nonflow) conditions. Isoelectric sample biological analytes, bovine hemoglobin serum albumin, was performed illustrate “microfluidic transverse IEF” for continuous concentration separation systems.