Genomic structure of a Y-specific ribonucleic acid binding motif-containing gene: a putative candidate for a subset of male infertility.

摘要: The genetic basis of infertility remains unclear in a majority infertile men. Deletion mapping studies suggest that genes on the long arm Y-chromosome (Yq) may be important spermatogenic process and play pathogenetic role subset Complementary DNA sequences two Y-specific contain ribonucleic acid binding motifs and, therefore, referred to as RBM (previously named YRRM) were published recently. To develop PCR-single strand conformation polymorphism strategy for detection point mutations gene(s) men, we determined genomic structure flanking at intron-exon junctions. Two separate strategies used parallel isolate fragment bearing gene. first employed screening P1 library using PCR primers corresponding 5'- 3'-ends RBM-1 complementary sequence. second subcloning YAC c...