Development of a Biosensor for Copper Detection in Aqueous Solutions Using an Anemonia sulcata Recombinant GFP

摘要: Fluorescent proteins from marine organisms represent potential candidates for biosensor development. In this paper, we described the isolation of a native green fluorescent protein Anemonia sulcata and cloning purification its equivalent as recombinant in Escherichia coli. Furthermore, spectroscopic behaviours GFPs were investigated function Cu2+, Cd2+, Pb2+ Ni2+ concentration. Our results suggest high selectivity both at copper than other metals and, protein, great sensitivity very low concentration (0.1–1 μM). Moreover, starting these data, using combination molecular biology techniques optical setup, developed device detection Cu2+ water solutions. The quenching effect detected with showed that relative attenuation signal (0.46 ± 0.02 AU) was slightly larger data measured by fluorescence spectra (0.65 ± 0.03 AU). good span two orders magnitude concentration, fact instrument is made up low-cost sturdy parts selective rAsGFP to ions make setup suited cost, on-the-field, ion-specific biosensor.