Telomere Maintenance Requires the RAD51D Recombination/Repair Protein
作者： Madalena Tarsounas , Purificacıon Munoz , Andreas Claas , Phillip G Smiraldo , Douglas L Pittman
DOI: 10.1016/S0092-8674(04)00337-X
关键词:
摘要: Abstract The five RAD51 paralogs (RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3) are required in mammalian cells for normal levels of genetic recombination resistance to DNA-damaging agents. We report here that RAD51D is also involved telomere maintenance. Using immunofluorescence labeling, electron microscopy, chromatin immunoprecipitation assays, was shown localize the telomeres both meiotic somatic cells. Telomerase-positive Rad51d −/− Trp53 primary mouse embryonic fibroblasts (MEFs) exhibited telomeric DNA repeat shortening compared or wild-type MEFs. Moreover, elevated chromosomal aberrations were detected, including end-to-end fusions, a signature dysfunction. Inhibition synthesis telomerase-negative immortalized human by siRNA resulted erosion chromosome fusion. conclude plays dual cellular role repair double-strand breaks protection against attrition
unirioja.es
elsevier.com
cnio.es 
sci-hub.se 参考文章(67)
Miguel Godinho Ferreira, Kyle M Miller, Julia Promisel Cooper, Indecent exposure: when telomeres become uncapped. Molecular Cell. ,vol. 13, pp. 7- 18 ,(2004) , 10.1016/S1097-2765(03)00531-8
Kum Kum Khanna, Stephen P. Jackson, DNA double-strand breaks: signaling, repair and the cancer connection. Nature Genetics. ,vol. 27, pp. 247- 254 ,(2001) , 10.1038/85798
Titia de Lange, Protection of mammalian telomeres Oncogene. ,vol. 21, pp. 532- 540 ,(2002) , 10.1038/SJ.ONC.1205080
Melissa A. Dunham, Axel A. Neumann, Clare L. Fasching, Roger R. Reddel, Telomere maintenance by recombination in human cells Nature Genetics. ,vol. 26, pp. 447- 450 ,(2000) , 10.1038/82586
Xu-Dong Zhu, Bernhard Küster, Matthias Mann, John HJ Petrini, Titia de Lange, None, Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres. Nature Genetics. ,vol. 25, pp. 347- 352 ,(2000) , 10.1038/77139
T. M. Bryan, A. Englezou, J. Gupta, S. Bacchetti, R. R. Reddel, Telomere elongation in immortal human cells without detectable telomerase activity. The EMBO Journal. ,vol. 14, pp. 4240- 4248 ,(1995) , 10.1002/J.1460-2075.1995.TB00098.X
Jeremy D Henson, Axel A Neumann, Thomas R Yeager, Roger R Reddel, Alternative lengthening of telomeres in mammalian cells Oncogene. ,vol. 21, pp. 598- 610 ,(2002) , 10.1038/SJ.ONC.1205058
H.-L. Hsu, D. Gilley, E. H. Blackburn, D. J. Chen, Ku is associated with the telomere in mammals Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 12454- 12458 ,(1999) , 10.1073/PNAS.96.22.12454
Peter B. Moens, David J. Chen, Zhiyuan Shen, Nadine Kolas, Madalina Tarsounas, Henry H. Q. Heng, Barbara Spyropoulos, Rad51 immunocytology in rat and mouse spermatocytes and oocytes. Chromosoma. ,vol. 106, pp. 207- 215 ,(1997) , 10.1007/S004120050241
Ralph Scully, Junjie Chen, Annemieke Plug, Yonghong Xiao, David Weaver, Jean Feunteun, Terry Ashley, David M Livingston, Association of BRCA1 with Rad51 in Mitotic and Meiotic Cells Cell. ,vol. 88, pp. 265- 275 ,(1997) , 10.1016/S0092-8674(00)81847-4