A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neurite outgrowth.
作者： K Tashiro , G C Sephel , B Weeks , M Sasaki , G R Martin
DOI: 10.1016/S0021-9258(18)71604-9
关键词:
摘要: Abstract Laminin is a basement membrane glycoprotein which consists of A, B1, and B2 chains. has diverse biological activities including promoting cell adhesion, migration, differentiation, growth, neurite extension. Synthetic peptides from the active region A chain were prepared tested for their activity. 19-mer peptide (designated PA22-2), just above carboxyl globule on long arm chain, was found to promote spreading, outgrowth. By testing smaller sequences within peptide, constituent pentapeptide, IKVAV (Ile-Lys-Val-Ala-Val), identified as site adhesion These data suggest that this sequence one principle sites in laminin regulate cellular behavior.
sci-hub.st 参考文章(43)
C N Rao, I M Margulies, T S Tralka, V P Terranova, J A Madri, L A Liotta, Isolation of a subunit of laminin and its role in molecular structure and tumor cell attachment. Journal of Biological Chemistry. ,vol. 257, pp. 9740- 9744 ,(1982) , 10.1016/S0021-9258(18)34135-8
Steady-state levels of mRNAs coding for the type IV collagen and laminin polypeptide chains of basement membranes exhibit marked tissue-specific stoichiometric variations in the rat. Journal of Biological Chemistry. ,vol. 262, pp. 12475- 12478 ,(1987) , 10.1016/S0021-9258(18)45230-1
M Sasaki, H K Kleinman, H Huber, R Deutzmann, Y Yamada, Laminin, a multidomain protein. The A chain has a unique globular domain and homology with the basement membrane proteoglycan and the laminin B chains. Journal of Biological Chemistry. ,vol. 263, pp. 16536- 16544 ,(1988) , 10.1016/S0021-9258(18)37424-6
R Timpl, S Johansson, Vera van Delden, I Oberbäumer, M Höök, None, Characterization of protease-resistant fragments of laminin mediating attachment and spreading of rat hepatocytes. Journal of Biological Chemistry. ,vol. 258, pp. 8922- 8927 ,(1983) , 10.1016/S0021-9258(18)32145-8
M Aumailley, V Nurcombe, D Edgar, M Paulsson, R Timpl, The cellular interactions of laminin fragments. Cell adhesion correlates with two fragment-specific high affinity binding sites. Journal of Biological Chemistry. ,vol. 262, pp. 11532- 11538 ,(1987) , 10.1016/S0021-9258(18)60840-3
R. Timpl, H. Rohde, P.G. Robey, S.I. Rennard, J.M. Foidart, G.R. Martin, Laminin--a glycoprotein from basement membranes. Journal of Biological Chemistry. ,vol. 254, pp. 9933- 9937 ,(1979) , 10.1016/S0021-9258(19)83607-4
S. L. Goodman, Two distinct cell-binding domains in laminin can independently promote nonneuronal cell adhesion and spreading. Journal of Cell Biology. ,vol. 105, pp. 589- 598 ,(1987) , 10.1083/JCB.105.1.589
Jeannette Graf, Yukihide Iwamoto, Makoto Sasaki, George R. Martin, Hynda K. Kleinman, Frank A. Robey, Yoshihiko Yamada, Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding Cell. ,vol. 48, pp. 989- 996 ,(1987) , 10.1016/0092-8674(87)90707-0
Y Iwamoto, F. Robey, J Graf, M Sasaki, H. Kleinman, Y Yamada, G. Martin, YIGSR, a synthetic laminin pentapeptide, inhibits experimental metastasis formation. Science. ,vol. 238, pp. 1132- 1134 ,(1987) , 10.1126/SCIENCE.2961059
Frank A. Robey, Raymond L. Fields, Automated synthesis of N-bromoacetyl-modified peptides for the preparation of synthetic peptide polymers, peptide-protein conjugates, and cyclic peptides. Analytical Biochemistry. ,vol. 177, pp. 373- 377 ,(1989) , 10.1016/0003-2697(89)90068-7