High-fat hypercaloric diet induces obesity, glucose intolerance and hyperlipidemia in normal adult male Wistar rat

摘要: There is strong evidence that genetic factors contribute to the development of obesity in humans as well laboratory animals. Another important factor leading an increase energy intake. However, it difficult make normal rats obese by controlling daily food no report adult male Wistar becoming and diabetic on a high-fat diet. The aim present study was, therefore, infusing high fat hypercaloric diet through cannula without disturbing free movement investigate influence caloric intake body weight glucose metabolism. High-fat (360 kcal/kg wt./day; H group) or control (180 C was continuously infused into stomach weighing approximately 300 g gastric cannulas for 27 days. On day 28 after 24-h fasting, serum concentrations aspartate aminotransferase, alanine total cholesterol, triglyceride, phospholipid, fatty acids (FFA) were determined, intragastric loading test (2 g/kg wt.) performed. average weekly gain group twice much (40.0 ± 2.4 vs. 19.4 1.9 g/week, P < 0.001). Serum levels FFA significantly elevated compared those group. Liver higher than showed steatosis. Pancreas (−13%) protein (−12%), amylase (−53%) trypsin content (−26%) all reduced, whereas pancreatic DNA increased insulin before Moreover, response relative group, indicating presence resistance. These results indicate feeding makes rat associated with hyperlipidemia, hyperinsulinemia, intolerance.