Cloning, mapping, and characterization of the human Rab3C gene.
作者： Haipeng Cheng , Yushu Ma , Xiaohua Ni , Min Jiang , Yao Luo
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摘要: Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells. A subset of proteins, the Rab3 thought to play an important role regulated exocytosis vesicles. During large-scale sequencing analysis a human fetal brain cDNA library, we isolated clone encoding novel protein, which showed 99% identity with previously bovine Rab3C at amino acid level. It contained four conserved motifs characteristic family. RT-PCR indicated was expressed brain, placenta, and lung. By mapping, localized gene chromosome 5q13. The consisted 6 exons spanning more than 310 kb genomic DNA. Rab3A, Rab3B, Rab3D have been mapped three different chromosomes, suggesting they not transcripts same gene.
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L. Johannes, P.M. Lledo, M. Roa, J.D. Vincent, J.P. Henry, F. Darchen, The GTPase Rab3a negatively controls calcium-dependent exocytosis in neuroendocrine cells. The EMBO Journal. ,vol. 13, pp. 2029- 2037 ,(1994) , 10.1002/J.1460-2075.1994.TB06476.X
Y Matsui, A Kikuchi, J Kondo, T Hishida, Y Teranishi, Y Takai, Nucleotide and deduced amino acid sequences of a GTP-binding protein family with molecular weights of 25,000 from bovine brain. Journal of Biological Chemistry. ,vol. 263, pp. 11071- 11074 ,(1988) , 10.1016/S0021-9258(18)37922-5
G. Fischer von Mollard, B. Stahl, A. Khokhlatchev, T.C. Südhof, R. Jahn, Rab3C is a synaptic vesicle protein that dissociates from synaptic vesicles after stimulation of exocytosis. Journal of Biological Chemistry. ,vol. 269, pp. 10971- 10974 ,(1994) , 10.1016/S0021-9258(19)78076-4
Martin Geppert, Yukiko Goda, Charles F. Stevens, Thomas C. Südhof, The small GTP-binding protein Rab3A regulates a late step in synaptic vesicle fusion Nature. ,vol. 387, pp. 810- 814 ,(1997) , 10.1038/42954
A Mizoguchi, S Kim, T Ueda, A Kikuchi, H Yorifuji, N Hirokawa, Y Takai, Localization and subcellular distribution of smg p25A, a ras p21-like GTP-binding protein, in rat brain. Journal of Biological Chemistry. ,vol. 265, pp. 11872- 11879 ,(1990) , 10.1016/S0021-9258(19)38480-7
G. Fischer von Mollard, G. A. Mignery, M. Baumert, M. S. Perin, T. J. Hanson, P. M. Burger, R. Jahn, T. C. Sudhof, rab3 is a small GTP-binding protein exclusively localized to synaptic vesicles. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 1988- 1992 ,(1990) , 10.1073/PNAS.87.5.1988
G. Baldini, T. Hohl, H. Y. Lin, H. F. Lodish, Cloning of a Rab3 isotype predominantly expressed in adipocytes. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 5049- 5052 ,(1992) , 10.1073/PNAS.89.11.5049
Pierre-Marie Lledo, Philippe Verniert, Jean-Didier Vincent, William T. Mason, Robert Zorec, Inhibition of Rab3B expression attenuates Ca(2+)-dependent exocytosis in rat anterior pituitary cells. Nature. ,vol. 364, pp. 540- 544 ,(1993) , 10.1038/364540A0
Ian G. Macara, Role of the Rab3A GTPase in regulated secretion from neuroendocrine cells. Trends in Endocrinology and Metabolism. ,vol. 5, pp. 267- 271 ,(1994) , 10.1016/1043-2760(94)P3201-H
H Ohnishi, L C Samuelson, D I Yule, S A Ernst, J A Williams, Overexpression of Rab3D enhances regulated amylase secretion from pancreatic acini of transgenic mice. Journal of Clinical Investigation. ,vol. 100, pp. 3044- 3052 ,(1997) , 10.1172/JCI119859