Validation of a Novel Fgf10Cre-ERT2 Knock-in Mouse Line Targeting FGF10Pos Cells Postnatally.

摘要: Fgf10 is a key gene during development, homeostasis and repair after injury. We previously reported knock-in Fgf10Cre-ERT2 line (with the Cre-ERT2 cassette inserted in frame with start codon of exon 1), called thereafter Fgf10Ki-v1, to target FGF10Pos cells. While this allowed fairly efficient specific labeling cells embryonic stage, it failed these birth, particularly postnatal lung, which has been focus our research. report here generation validation new (called Fgf10Ki-v2) insertion expression stop 3. Fgf10Ki-v2/+ heterozygous mice exhibited comparable levels wild type animals. However, mismatch between Cre was observed lungs. In addition, lung limb agenesis were homozygous embryos suggesting loss functional allele Fgf10Ki-v2 mice. Bioinformatic analysis shows that 3'UTR, where inserted, contains numerous putative transcription factor binding sites. By crossing tdTomato reporter line, we demonstrated faithfully recapitulated development. Importantly, mouse capable significantly targeting adult lung. Therefore, despite aforementioned limitations, opens way for future mechanistic experiments involving