Methods of screening for compounds having anti-inflammatory activity
作者： Sonja Reingruber , Waltraud Pasteiner , Marion Groger , Rainer Henning , Peter Petzelbauer
DOI:
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摘要: A method screens compounds out of the group consisting proteins, peptides, peptidomimetics, antibodies and small organic molecules that increase activity protein Rac1 by virtue their binding to extracellular portion this protein. The includes steps of: contacting a confluent layer cultured endothelial cells with at least one test compounds, lysing lysation buffer, measuring amount activity.
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