A Chemical Counterpunch: Chromobacterium violaceum ATCC 31532 Produces Violacein in Response to Translation-Inhibiting Antibiotics.

摘要: ABSTRACT Antibiotics produced by bacteria play important roles in microbial interactions and competition Antibiosis can induce resistance mechanisms target organisms, at sublethal doses, antibiotics have been shown to globally alter gene expression patterns. Here, we show that hygromycin A from Streptomyces sp. strain 2AW. induces Chromobacterium violaceum ATCC 31532 produce the purple antibiotic violacein. Sublethal doses of other similarly polypeptide elongation step translation likewise induced violacein production, unlike with different targets. C. biofilm formation virulence against Drosophila melanogaster were also translation-inhibiting antibiotics, identified an antibiotic-induced response (air) two-component regulatory system is required for these responses. Genetic analyses indicated a connection between Air system, quorum-dependent signaling, negative regulator VioS, leading us propose model induction production. This work suggests novel mechanism interspecies interaction which bacterium produces inhibition another supports role as signal molecules. IMPORTANCE Secondary metabolites communities, but their natural functions are often unknown may be more complex than appreciated. While compounds activity assumed underlie competition, they alternatively act In either scenario, microorganisms might evolve responses concentrations metabolites, protect themselves or change certain behaviors local abundance species. report production 2AW, this dependent on translational previously uncharacterized system. The breadth transcriptional beyond surprisingly metabolite-mediated microbe-microbe hypothesis evolved These insights will inform predictive models soil community dynamics unintended effects clinical administration.