In Vitro Model Systems to Investigate Drug Resistance Mechanisms in Pancreatic Cancer Cells

摘要: With a 5-year survival rate of less than 6%, the diagnosis pancreatic cancer is devastating news for any patient. Gemcitabine, most commonly used chemotherapy drug, only improves by approximately 1.5 months. A major obstacle to treatment with gemcitabine development drug resistance. To better understand precise mechanisms which patient tumor cells gain resistance gemcitabine, cell culture model system that more accurately reflects in vivo required. In this study, cultured adenocarcinoma BxPC-3 were subjected two different regimens. The first method—termed pulse method—involves periodically treating separate cultures constant predetermined doses gemcitabine. second regimen—termed incremental method—consists increasing from 10 100 nM. While all treated showed enhanced low-dose treatments sufficient produce highly drug-resistant as evidenced higher IC50 measurements. Pulse also resulted slower growth rates and increased doubling time cells. Morphological changes indicate cellular abnormalities linked likely epithelial-to-mesenchymal transition Our preliminary results method may simulate observed patients undergoing serve superior investigate drug-resistance. This can help identification appropriate markers determine presence clinicians adjust strategies improve outcomes suffering cancer.