The seminal fluid proteome of the honeybee Apis mellifera

摘要: Ejaculates contain sperm but also seminal fluid, which is increasingly recognized to be of central importance for reproductive success. However, a detailed biochemical composition and physiological understanding fluid still elusive. We have used MS identify the 57 most abundant proteins within ejaculated honeybee Apis mellifera. Their amino acid sequences revealed presence diverse functional categories enzymes, regulators structural proteins. A number known or predicted roles in maintaining viability, protecting from microbial infections interacting with physiology female. range putative glycoproteins glycosylation enzymes were detected among 57, subsequent fluorescent staining glycolysation several prominant while no samples. Many that accumulate did not predictable tags secretion cell. Comparison Drosophila (including secreted accessory gland as ACPs), human proteome bee protein set contains newly identified we noted more similarity current than The thus represents an important addition available data comparative studies proteomes insects.