Effects of in vitro exposures to cadmium, mercury, zinc, and 1-(2-chlorophenyl)-1-(4-chlorophenyl)-2,2-dichloroethane on steroidogenesis by dispersed interrenal cells of rainbow trout (Oncorhynchus mykiss).

摘要: Abstract Numerous environmental xenobiotics can act as endocrine disrupters in wildlife species. Fish chronically exposed to pollutants exhibit a deficiency the synthesis of cortisol, glucocorticosteroid hormone secreted by interrenal steroidogenic cells response ACTH mechanism mediated cAMP. The capacity series heavy metals (CdCl 2 , ZnCl HgCl and CH 3 HgCl) 1-(2-chlorophenyl)-1-(4-chlorophenyl)-2,2-dichloroethane (o,p′-DDD) disrupt cortisol secretion was determined dispersed rainbow trout ( Oncorhynchus mykiss ) vitro toxicant, measuring stimulated with or dibutiryl-cAMP (dbcAMP) assessing cell viability. effect cadmium presence zinc on also determined. median lethal concentration (LC50, dose that kills 50% cells), effective (EC50, inhibits 50%), LC50/EC50 ratio were for each chemical compare its toxicity test specificity toxicants disrupters. had lowest EC50 LC50; it most toxic chemicals tested. highest CdCl indicating these specific toxicity. cortisol-secreting involves site situated downstream from step generating cAMP, while o,p′-DDD seemed impair located between adenyl cyclase activation binding. No evidence protector against found.