Mixed mode monolithic sorbent in pipette tip for extraction of ractopamine and clenbuterol prior to analysis by HPLC-UV and UHPLC-Q Exactive TM Plus Orbitrap MS
作者： Nakin Surapanich , Chanpen Karuwan , Duangjai Nacapricha , Prapin Wilairat , Patcharin Chaisuwan
DOI: 10.1186/S40543-021-00275-5
关键词:
摘要: This work developed a fast and simple method for the quantification of two important β-agonists, ractopamine (RAC) clenbuterol (CLEN) in animal urines. The was based on micro-extraction by mixed mode monolithic material analysis HPLC-UV UHPLC-Q ExactiveTM Plus Orbitrap mass spectrometer. A functional monolith with nonpolar ester-based structure containing polar carboxylic groups thermally synthesized situ pipette tips. combines both hydrophobic ionic interactions. Parameters including conditions composition reagents in-pipette tip synthesis, as well extraction process, were investigated optimized. procedure is 800 μL washing solvent 10:90 (v/v) ACN:water 150 eluting 30:70 ACN:200 mM acetate buffer pH 4.0. Extraction efficiencies 92% 100% RAC CLEN, respectively, achieved within 5 min total organic consumption 395 μL. extracts spiked cattle swine urines analyzed Good recovery acceptable precision observed. spectrometry data confirmed efficient matrix removal sorbent allowing routine method.
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