Effect of UV irradiation on Sulfolobus acidocaldarius and involvement of the general transcription factor TFB3 in the early UV response.

摘要: Exposure to UV light can result in severe DNA damage. The alternative general transcription factor (GTF) TFB3 has been proposed play a key role the stress response thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. Reporter gene assays confirmed that tfb3 is upregulated 90–180 min after treatment. In vivo tagging and immunodetection of induced expression at 90 min. Analysis insertion mutant showed genes encoding proteins Ups pili Ced importer are no longer treatment, which was by aggregation assays. Thus, plays crucial activation these genes. Genome wide transcriptome analysis allowed differentiation between TFB3-dependent TFB3-independent early response. characterized induction TFB3, followed involved e.g. formation importer. Many were downregulated confirming hypothesis acts as an activator transcription. includes repression nucleotide metabolism, replication cell cycle progression order allow repair.