Detecting nuisance species using NGST: Methodology shortcomings and possible application in ballast water monitoring

摘要: Detecting the presence of potential invasive species in ballast water is a priority for preventing their spread into new environments. Next generation sequencing technologies are being increasingly used exploring and assessing biodiversity from environmental samples. Here we apply high throughput DNA extracted (BW) samples employing two different platforms, Ion Torrent 454, compare putative catalogues resulting Operational Taxonomic Units (OTU). Water were taken RV Polastern tank five days between second twentieth navigation day. Pronounced decrease oxygen concentration increase temperature occurred BW during this time, coincident with progressively higher proportion unassigned OTU short reads indicating degradation. Discrepancy platforms was consistent previously published bias AT-rich sequences platform. Some detected increased frequency Polarstern travel, which suggests they alive therefore tolerant to adverse conditions. assigned highly red alga Polysiphonia have been at low but increasing platforms. Although moment NGST could not replace current methods sampling, sorting individual taxonomic identification biota, it has as an exploratory methodology especially detecting scarce species.