摘要: Structural studies provide insight into the mechanisms governing a checkpoint in cell division that prevents chromosomes from segregating before they are properly aligned on structure called mitotic spindle. See Article p.431 The spindle assembly (SAC) is surveillance mechanism detects incorrect chromatid kinetochore attachments and delays chromosome segregation by generating 'wait anaphase' signal. It activated via complex (MCC), which inhibits anaphase-promoting (APC/C), multimeric E3 ligase. Here, David Barford colleagues use cryo-electron microscopy to determine near-atomic resolution structures of APC/C–MCC complex. reveal how MCC interacts with represses APC/C obstructing substrate recognition suppressing ligase activity.
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