Mapping free-standing homing endonuclease promoters using 5'RLM-RACE.
作者： Ewan A. Gibb
DOI: 10.1007/978-1-62703-968-0_5
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摘要: 5'RLM-RACE is a PCR-based technique used to map the 5' termini of transcripts in both eukaryotic and prokaryotic organisms. Free-standing homing endonuclease promoters often lack recognizable making predicting transcriptional start site challenging. Furthermore, endonucleases are expressed at very low levels transcript mapping challenge. Here, I present protocol with special considerations for expected abundance their potential be subjected RNA processing events.
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