Secretive Expression of Insect Antifungal Peptide-Encoded Genes in Pichia pastoris and Activity Assay of the Products
作者： Yan-xia SANG , Xiao-juan DENG , Wan-ying YANG , Wen-xian WANG , Shuo-yang WEN
DOI: 10.1016/S1671-2927(07)60165-5
关键词:
摘要: Abstract The antifungal peptides, drosomycin (Drs) and its isoform drosomycin-like C (Drs-IC) from Drosophila melanogaster Thanatin Podisus maculiventris , have potent activity with broad spectrum against filamentous fungi. Secretive expression of these genes in yeasts makes it possible to utilize the supernatants yeast culture as protective reagents on fruit, vegetable, food other agricultural products. So study effective secretion by system is great importance. Three genes, Drs, Drs-IC were cloned into pPICZαA recombinant vectors, pPICZαA-Drs, pPICZαA-Drs-IC, pPICZαA-Thanatin transformed Pichia pastoris electric transfer method. P. which was screened phenotype selection PCR amplification, induced express peptide methanol. expressive products three recombinants showed 5 out 6 test fungi strains, also had strong tested bacteria. constructed pastoris. expressed peptides successfully secreted medium exhibited activities strains.
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