Xist repression shows time-dependent effects on the reprogramming of female somatic cells to induced pluripotent stem cells.
作者： Qi Chen , Shuai Gao , Wenteng He , Xiaochen Kou , Yanhong Zhao
DOI: 10.1002/STEM.1775
关键词:
摘要: Although the reactivation of silenced X chromosomes has been observed as part process reprogramming female somatic cells into induced pluripotent stem (iPSCs), it remains unknown whether repression X-inactive specific transcript (Xist) can greatly enhance iPSC induction similar to that in cell nuclear transfer studies. In this study, we discovered Xist plays opposite roles early and late phases iPSCs induction. Our results demonstrate downregulation by an isopropyl β-d-1-thiogalactopyranoside (IPTG)-inducible short hairpin RNA (shRNA) system impair mesenchymal-to-epithelial transition (MET) phase but significantly promote pre-iPSCs phase. Furthermore, although knockdown did not affect H3K27me3 modification on chromosome, macroH2A was released from inactivated chromosome (Xi). This enables silencing be a reversible event. Moreover, supplementation vitamin C (Vc) augment stabilize preventing relocalization Xi. Therefore, our study reveals role stages pluripotency demonstrates release iPSCs.
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