Cloning and Characterization of Two cDNA Sequences Coding Squalene Synthase Involved in Glycyrrhizic Acid Biosynthesis in Glycyrrhiza uralensis
作者： Ying Liu , Ning Zhang , Honghao Chen , Ya Gao , Hao Wen
DOI: 10.1007/978-94-007-7618-0_32
关键词:
摘要: Glycyrrhiza uralensis are widely used in Chinese medicine for the functions of relieving cough, clearing heat and detoxicating. They also as food additives industry materials. There various components with different pharmacological activities G. uralensis, among them glycyrrhizic acid is believed an indicator component to characterize quality this herb. In present study, two cDNA sequences coding squalene synthase (SQS1 SQS2) involved biosynthesis were cloned expressed Escherichia coli fusion proteins, which showed similar activity SQS isolated from other species catalyze farnesyl diphosphate (FPP) into (SQ). Because a key enzyme metabolic pathway work significant further studies concerned exploring molecular mechanism improving uralensis.
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