Determination of Monosaccharides, Disaccharides, and Oligosaccharides in the Same Plant Sample by High-Performance Liquid Chromatography

摘要: A method for determining low-molecular carbohydrates based on the use of HPLC with refractometric detection was modified. In contrast to previous methods, determination qualitative and quantitative composition sugars in plant extracts performed without their preliminary separation into monosaccharides, disaccharides, oligosaccharides. The exclusion an additional stage made it possible reduce twofold time analysis a single sample, save expensive materials necessary carbohydrate HPLC, increase useful life analytical column.